vTAG™ ANTI-HUMAN CD37 ANTIBODY
NO WASH, QUANTITATIVE CD37 MEASUREMENT BY vFC™
$115.00 – $295.00
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Conjugate(s): FITC
Est. Limit of Detection: 30 Molecules per EV
Cargo analysis using vTag™ antibodies should be accompanied by a vesicle specific analysis method such as vFC™. vTag™ antibodies bundled with the vFC™ assay will be discounted 25%. |
Certificate of Analysis
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Description
CD9 Immunostaining in vFC™
vFC™ (#CBS4) is an assay that enables specific detection and characterization of individual extracellular vesicles such as exosomes and microvesicles on commercially available flow cytometers. This protocol makes use of vFC™ to provide a standardized means of analyzing individual vesicles to measure size, concentration, and CD37 expression simultaneously. Because vFC™ triggers on vesicles, pairing antibody staining with vFC™ enables a highly-reproducible, no-wash assay. In this protocol, vesicles will be stained using our vTag™ Anti-Human CD37 Antibody. See protocol 2 in the vFC™ kit handbook for additional considerations and full protocol details.Procedure
- Dilute sample containing EVs to approximately 5×106 extracellular vesicles per µl. To empirically determine vesicle concentration and identify an optimal pre-stain dilution, see supporting protocols included in the vFC™ kit.
- Prepare a working concentration of vFRed™ Red (5 µl per well) according to kit instructions
- In duplicate, in the included 96 well plate, add 35 µl of dilution buffer, 5µl of vFRed™ Red, 5 µl of vTag™ anti-human CD37 antibody, and 5 µl of EV sample.
- Incubate in the dark for 1hr.
- Perform serial dilution of the samples as described in the assay handbook. Briefly, samples are to be diluted from between 1:100 to 1:1000 from staining concentration depending upon the flow cytometer being used.
- Analyze the diluted sample of labeled EVs on a qualified flow cytometer.
CD37 is a tetraspanin protein primarily expressed on the surface of B cells, macrophages, and dendritic cells. It plays a crucial role in immune system regulation by forming complexes with other proteins in the tetraspanin web, influencing cell signaling, adhesion, and migration. CD37 is particularly important in modulating the adaptive immune response, assisting in the activation of T cells and the proper functioning of antigen-presenting cells. Deficiencies in CD37 have been linked to impaired immune responses and can affect processes like B-cell maturation and antibody production. (see the data by vesicle origin resource for more info).
MSDS-
vFC™ VESICLE FLOW CYTOMETRY EV ANALYSIS ASSAY
$450.00 – $2,100.00 Select options This product has multiple variants. The options may be chosen on the product page
FOR COUNTING AND SIZING VESICLES IN BIOFLUIDS, MEDIA, OR BUFFER -
HUMAN PLATELET DERIVED EXTRACELLULAR VESICLES
$275.00 – $1,300.00 Select options This product has multiple variants. The options may be chosen on the product page
Additional information
| Size | 20 Tests, 100 Tests |
|---|---|
| Conjugate |
NO WASH, QUANTITATIVE CD37 MEASUREMENT BY vFC™”
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