vTAG™ ANTI-HUMAN CD82 ANTIBODY
NO WASH, QUANTITATIVE CD82 MEASUREMENT BY vFC™
$105.00 – $315.00
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Conjugate(s): PE
Est. Limit of Detection: 30 Molecules per EV
Cargo analysis using vTag™ antibodies should be accompanied by a vesicle specific analysis method such as vFC™. vTag™ antibodies bundled with the vFC™ assay will be discounted 25%. |
Certificate of Analysis
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Description
CD82 Immunostaining in vFC™
vFC™ (#CBS4) is an assay that enables specific detection and characterization of individual extracellular vesicles such as exosomes and microvesicles on commercially available flow cytometers. This protocol makes use of vFC™ to provide a standardized means of analyzing individual vesicles to measure size, concentration, and CD82 expression simultaneously. Because vFC™ triggers on vesicles, pairing antibody staining with vFC™ enables a highly-reproducible, no-wash assay. In this protocol, vesicles will be stained using our vTag™ Anti-Human CD82 Antibody. See protocol 2 in the vFC™ kit handbook for additional considerations and full protocol details.Procedure
- Dilute sample containing EVs to approximately 5×106 extracellular vesicles per µl. To empirically determine vesicle concentration and identify an optimal pre-stain dilution, see supporting protocols included in the vFC™ kit.
- Prepare a working concentration of vFRed™ Red (5 µl per well) according to kit instructions
- In duplicate, in the included 96 well plate, add 35 µl of dilution buffer, 5µl of vFRed™ Red, 5 µl of vTag™ anti-human CD82 antibody, and 5 µl of EV sample.
- Incubate in the dark for 1hr.
- Perform serial dilution of the samples as described in the assay handbook. Briefly, samples are to be diluted from between 1:100 to 1:1000 from staining concentration depending upon the flow cytometer being used.
- Analyze the diluted sample of labeled EVs on a qualified flow cytometer.
CD82, also known as KAI1, is a member of the tetraspanin family and is primarily involved in cell adhesion and signaling. This protein plays a role in regulating cell growth, motility, and metastasis in cancer cells. CD82 interacts with integrins and other proteins, influencing cell migration, immune response, and tumor suppression. It is widely expressed in various tissues and is often studied for its potential in cancer prognosis due to its association with tumor progression and metastasis suppression.
In the context of extracellular vesicles (EVs), CD82 is prominently involved in the formation and secretion of exosomes, a subtype of EVs. Exosomes enriched in CD82 carry important signaling molecules, proteins, and RNA that can be transferred between cells, contributing to intercellular communication. These vesicles are involved in various physiological and pathological processes, such as immune regulation and cancer progression. CD82-containing EVs help mediate the transfer of cargo to recipient cells, impacting processes such as cell adhesion, immune signaling, and the metastatic potential of tumor cells. (see the data by vesicle origin resource for more info).
MSDS-
vFC™ VESICLE FLOW CYTOMETRY EV ANALYSIS ASSAY
$450.00 – $2,100.00 Select options This product has multiple variants. The options may be chosen on the product page
FOR COUNTING AND SIZING VESICLES IN BIOFLUIDS, MEDIA, OR BUFFER -
HUMAN PLATELET DERIVED EXTRACELLULAR VESICLES
$275.00 – $1,300.00 Select options This product has multiple variants. The options may be chosen on the product page
Additional information
| Size | 20 Tests, 100 Tests |
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| Conjugate | PE, APC |
NO WASH, QUANTITATIVE CD82 MEASUREMENT BY vFC™”
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