Description
vCal™ Antibody Capture nanoBeads are calibrated antibody capture beads designed to assess and optimize antibodies for use in vesicle flow cytometry (vFC™). These beads bind all antibody isotypes and produce 3 peaks corresponding to 0, 200, and 1000 bound antibodies. Select your specificity based on the host of the antibody you are using. The 800nm size is suitable for detection on cytometers with lower scatter sensitivity allowing assessment of an instruments sensitivity for fluorescence detection of a particular antibody in a relevant range for vesicle immunostaining.
In general, for smaller vesicles it is important that an instrument is able to resolve all three peaks. For larger vesicles or very abundantly expressed proteins, being able to distinguish between the top two peaks may be sufficient for some analyses.
Species or Isotype: Select appropriate beads based on host species of your antibody. Mouse, Rabbit, Rat, and Hamster available.
Bead Population(s): 4 populations of beads. See certificate of analysis for number of antibody binding sites on each population.
Concentration: 1×106 beads per ml.
Bead Size: 800 nm.
Storage Buffer: 0.1um filtered PBS +0.09% sodium azide.
Storage Conditions: Store beads at 4°C. See vial for expiration date.
Additional information
Size | 2.5 ml |
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