Nanotracking Analysis (NTA) of Platelet Derived EV Reference Sample (CBS2)

Phosphatidylserine is a membrane marker present on most extracellular vesicles including exosomes and microvesicles. Extracellular vesicles isolated from platelets derived from patient samples were analyzed for presence of PS via VFC. Phycoerythrin (PE) labeled Annexin V or buffer only samples were prepared for VFC analysis. Data demonstrate strong PE fluorescence for the individual vesicles measured.

CD41 is a membrane marker present on most extracellular vesicles derived from human platelets. Extracellular vesicles including exosomes and microvesicles isolated from platelets derived from patient samples were analyzed for presence of CD41 via VFC. Phycoerythrin (PE) labeled anti-CD41 antibody or buffer only samples were prepared for VFC analysis. Data demonstrate the presence of high levels of CD41 on most vesicles in the samples measured.

HUMAN PLATELET DERIVED EXTRACELLULAR VESICLES

$275.00 – $1,300.00

Summary

Source:Human Platelets Vesicle Size:140 nm (Range: 80-210nm) About Our Standard Package Sizes		Certificate of Analysis
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SKU: CBS2 Categories: Standardized Extracellular Vesicle Reference Samples, Vesicles and Vesicle Standards Tags: human, Platelets, Vesicle Reference Sample

Description

Are these the right standards for my application?

Human platelet derived extracellular vesicles from Cellarcus provide a consistent, rigorously characterized reference sample to baseline assays over multiple runs. These samples are also suitable for researchers examining platelet derived exosomes, microvesicles, or other EVs.

Cellarcus’ Human Platelet Derived Extracellular vesicles are a reference sample prepared from platelets obtained by a standard platelet enrichment protocol. Platelet vesiculation is induced by Ca²⁺ selective ionophore. The resulting vesicles are isolated via centrifugation and provided at a concentration of 5 x 10⁶ vesicles/µL as measured by Vesicle Flow Cytometry (vFC™). Unlike traditional applications like NTA or TRPS, it is vesicle specific. The specificity of the measurement is a key driver behind 5-fold improved assay variability compared to these other applications. Concentrations measured by vFC™ are generally 1-2 log units lower than other applications because it is counting only vesicles, not all particles. vFC™ analysis enables characterization of these vesicles samples by size, concentration and cell surface cargo.

Available Sizes:50 µl 100 µl 500 µl

Species: Human

Source: Platelets from patient samples

Surface Molecule Expression Information: Positive: CD41, Phosphotidylserine (as labeled by Annexin V).
Below Limit of Detection: (none tested).

Test and Concentration Information:Human Platelet Derived Extracellular Vesicles are EVs prepared by ionophore treatment of washed PLTs. This vesicle reference preparation is provided at a concentration of ~5 x 10⁶ vesicles/µL, as measured by Vesicle Flow Cytometry (VFC). PLT EVs are a unimodal population of EVs with a median diameter of ~140 nm and a range of 80-210 nm, as measured by VFC.

Vesicle Size: 140 nm (Range: 80-210nm)

Tested Applications: VFC, NTA (e.g. NanoSight^TM), RPS (e.g. qNano^TM)

Application Notes:

Vesicle flow cytometry requires a sufficiently sensitive instrument and vesicle analysis procedure. We provide simple, accurate, reproducible vesicle staining assays for this purpose (coming soon). At 5 x 10⁶ vesicles/µL this reference sample uses 5 µl/test for VFC analysis. The sample concentration requirement is the same for NTA or RPS, however, volumes required for assay on many instruments are up to 10-fold higher. As much as 50 µl of this EV reference sample may be required for these assays. Exact volume(s) of reagents should always be empirically determined.

Method of Preparation: Ionophore treatment of platelets followed by centrifugation to isolate vesicles.

Buffer: Well-filtered isotonic saline (e.g. PBS pH 7.4)

Storage Conditions: These vesicles are stable for up to 12 months when stored at -80°C. Vesicles may also be stored cold (e.g.4°C) for up to 2 weeks.

Safety Considerations: Material is derived from human blood. Please handle in accordance with CDC Biosafety Requirements and follow Universal Precautions for handling potentially infectious samples.

Additional information

Size	50 µl, 100 µl, 500µl
Parent SKU	CBS2

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	Phone: (858) 239-2100 Email: cellarcus@cellarcus.com