Lipo100 NanoTracking Analysis (NTA) Data

Phosphotidylserine is incorporated into the membrane of Lipo100™ Vesicle Size Standards upon synthesis. These vesicles were then stained with Phycoerythrin (PE) labeled Annexin V or left unstained for VFC analysis. Data demonstrate strong PE fluorescence for the individual vesicles measured.

LIPO100™ SYNTHETIC VESICLE SIZE STANDARD^ⓘ

$229.00 – $750.00

Summary

Source:Synthetic Vesicle Size:120 nm (Range: 80-140nm) About Our Standard Package Sizes		Certificate of Analysis
		TDS	MSDS

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SKU: CBS1 Category: Vesicles and Vesicle Standards

Description

Are these the right standards for my application?

For sizing vesicles like exosomes or microvesicles via standard methods such as NTA, RPS, or flow cytometry, Synthetic Vesicle Size Standards provide a well characterized synthetic vesicle population for assessing the accuracy of the vesicle size calculations.

Conventional standards used in vesicle sizing are polystyrene beads. PS beads are very different in their composition compared to natural vesicles and these differences lead to large underestimatation of vesicles’ size by as much as 40x. Cellarcus’ Synthetic Vesicle Size Standard is made of lipid vesicles called liposomes. These liposomes are prepared with lipid composition similar to that of a mammalian cell, extruded through a 100 nm nucleopore filter, and provided at a concentration of 5 x 10⁵ vesicles/µL as measured by Vesicle Flow Cytometry. The vesicle standard is a unimodal vesicle population with a median diameter of ~120 nm and a range of ~80-140 nm (as measured by NTA and RPS).

Available Sizes: 20 Tests 40 Tests 100 Tests

Species: Synthetic (Appropriate size standard for vesicles from mammalian species)

Source: Synthetic

Surface Molecule Expression Information: Phosphatidylserine (PS) was incorported during synthesis and confirmed via VFC testing with fluorescently labeled Annexin V.

Test and Concentration Information:Synthetic vesicles are supplied at a total of 5 x 10⁵ vesicles per µl (as measured by VFC). This equates to 5 µl per test. A test is defined as the number of vesicles typically used in Vesicle Flow Cytometry.

Vesicle Size: 120 nm (Range: 80-140nm)

Tested Applications: Vesicle Flow Cytometry (VFC), NTA (e.g. NanoSight^TM), RPS (e.g. qNano^TM)

Application Notes:

Vesicle flow cytometry requires a sufficiently sensitive instrument and vesicle analysis procedure. We provide simple, accurate, reproducible vesicle staining assays for this purpose (coming soon). Standards are titrated at 5 µl/test for VFC. For NTA or RPS, the same concentration of sample is generally sufficient, however, volumes required for assay on many instruments are up to 10-fold higher. As much as 50 µl of synthetic vesicles in solution may be required to standardize this approach when estimating vesicle sizes. Exact volume(s) of reagents should always be empirically determined.

Method of Preparation: 100 nm nucleopore SEC

Buffer: Well-filtered isotonic saline (e.g. PBS pH 7.4)

Storage Conditions: Synthetic Vesicle Size Standards are stable for up to 12 months when stored at 4°C.

Safety Considerations: Gloves should be worn at all times when handling this product.

Additional information

Size	20 Tests, 40 Tests, 100 Tests, 4 x 20 Tests

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📍	505 Coast Blvd S. Ste 409 La Jolla, CA USA 92037
	Phone: (858) 239-2100 Email: cellarcus@cellarcus.com

LIPO100™ SYNTHETIC VESICLE SIZE STANDARDⓘ