vCAL™ nanoCAL™ ANTIBODY CAPTURE BEADS
$265.00 – $350.00
Certificate of Analysis
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Description
vCal™ Antibody Capture nanoBeads are calibrated antibody capture beads designed to assess and optimize antibodies for use in vesicle flow cytometry (vFC™). These beads bind all antibody isotypes and produce 4 peaks with different intensities for instrument calibration. Calibrating your instrument enables you to report quantitative surface marker phenotypes which is important in comparing data across labs and time as well as for compliance with guidelines.
Select your specificity based on the host of the antibody you are using or for simpler workflows select pre-stained beads. The 800nm size is suitable for detection on cytometers with moderate scatter sensitivity.
In general, for smaller vesicles or dim markers it is important that an instrument is able to resolve all four peaks. For larger vesicles or very abundantly expressed proteins, being able to distinguish between brighter peaks may be sufficient for some analyses.
Species or Isotype: Select appropriate beads based on host species of your antibody. Mouse, Rabbit, Rat, and Hamster available.
Bead Population(s): 4 populations of beads. See certificate of analysis for number of antibody binding sites on each population.
Concentration: 1×106 beads per ml.
Bead Size: 800 nm.
Storage Buffer: 0.1um filtered PBS +0.09% sodium azide.
Storage Conditions: Store beads at 4°C. See vial for expiration date.
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Additional information
Size | 5 Tests, 25 Tests |
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Species Reactivity | Prelabeled, Mouse, Rat, Rabbit, Hamster, Human |
Fluorophore (Prelabeled Beads) | Unlabeled, V420, V510, V605, FITC, GFP, B520, PE, PE-Dazzle™, PE-Cy7, APC, R670 |
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